Isolation of cellular material under microscopic visualization using an adhesive/extraction reagent tipped probe

US5843644A · kind A · utility

Assignee

• The United States of America as Represented by the Secretary of the Department of Health and Human Services · US

Inventors

• Lance A. Liotta · Bethesda, US
• Zhengping Zhuang · Bethesda, US
• Michael R. Buck · Bethesda, US
• William G. Stetler-Stevenson · Kensington, US
• Irina A. Lubensky · Silver Spring, US
• Mark Roth · Appleton, US

Key dates

• Filing date: Mar 1, 1994
• Grant date: Dec 1, 1998
• Expiry date: Mar 1, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/805
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method of direct extraction of cellular material from a tissue sample which involves: forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extracted zone(s) of cells is subjected to analysis. The overall process of identifying, extracting, transporting, and analyzing the extracted zones(s) of cells can be fully automated.