Method for hypermutagenesis of nucleotides
US5843730A · kind A · utility
7Cited by
0References
45Claims
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Assignee
Inventors
Key dates
| Filing date | May 19, 1995 |
| Grant date | Dec 1, 1998 |
| Priority date | — |
| Expiry date | May 19, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/16122
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention features a method for introducing hypermutations into a target DNA or RNA sequence of interest, characterized in that said method comprises the steps of: PA1 (a) transcribing a RNA into DNA in a reaction mixture comprising a reverse transcriptase, varying biased concentrations of deoxynucleoside triphosphates to produce hypermutations and an oligonucleotide primer that is partially complementary to the 3' end of said RNA; and PA1 (b) recovering said DNA sequences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.