Identification of novel substrates
US5846765A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 16, 1995 |
| Grant date | Dec 8, 1998 |
| Priority date | — |
| Expiry date | May 16, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/75
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A method for identifying and selecting novel substrates for enzymes is provided. The method comprises constructing a gene fusion comprising DNA encoding a polypeptide fused to DNA encoding a substrate peptide, which in turn is fused to DNA encoding at least a portion of a phage coat protein. The DNA encoding the substrate peptide is mutated at one or more codons thereby generating a family of mutants. The fusion protein is expressed on the surface of a phagemid particle and subjected to chemical or enzymatic modification of the substrate peptide. Those phagemid particles which have been modified are then separated from those that have not.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.