Method of expressing antifreeze proteins in yeast
US5849537A · kind A · utility
3Cited by
3References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 12, 1994 |
| Grant date | Dec 15, 1998 |
| Priority date | — |
| Expiry date | Jan 12, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Yeast is genetically engineered by transformation with an expression vector containing a natural yeast secretion signal sequence combined appropriately with a chemically synthesized gene encoding antifreeze protein resulting in the expression, proper processing, and secretion of antifreeze protein which is heterologous to yeast in recoverable amounts. Disclosed are DNA sequences comprising structural genes encoding peptides having amino acid sequences with the biochemical or physiochemical properties of antifreeze protein and a method of combining the antifreeze protein gene sequences with appropriate expression vectors.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.