Linker for linked fusion polypeptides
US5856456A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 7, 1994 |
| Grant date | Jan 5, 1999 |
| Priority date | — |
| Expiry date | Apr 7, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/31
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention is directed to a novel peptide linker useful for connecting polypeptide constituents into a novel linked fusion polypeptide. The peptide linker of the invention provides greater stability and is less susceptible to aggregation than previously known peptide linkers. The peptide linker of the invention may be up to about 50 amino acids in length and contains at least one occurrence of a charged amino acid followed by a proline. When used for making a single chain Fv (sFv), the peptide linker is preferably from 18 to about 30 amino acids in length. A preferred embodiment of the peptide linker of the invention comprises the sequence: PA1 GSTSGSGXPGSGEGSTKG (SEQ. ID NO 1), where X is a charged amino acid, preferably lysine or arginine. Methods of making linked fusion polypeptides using the peptide linker of the invention are claimed. DNA molecules encoding such linked fusion polypeptides, and methods of producing such linked fusion polypeptides from these DNA molecules are also claimed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.