Methods, nucleotide sequences and host cells for assaying exogenous and endogenous protease activity
US5861267A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 1, 1995 |
| Grant date | Jan 19, 1999 |
| Priority date | — |
| Expiry date | May 1, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/545
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to methods for assaying exogenous protease activity in a host cell transformed with nucleotide sequences encoding that protease and a specialized substrate. It also relates to methods for assaying endogenous protease activity in a host cell transformed with nucleotide sequences a specialized substrate. When these nucleotide sequences are expressed, the exogenous or endogenous protease cleaves the substrate and releases a polypeptide that is secreted out of the cell, where it can be easily quantitated using standard assays. The methods and transformed host cells of this invention are particularly useful for identifying inhibitors of the exogenous and endogenous proteases. If the protease is a protease from an infectious agent, inhibitors identified by these methods are potential pharmaceutical agents for the treatment or prevention of infection by that agent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.