Assay and kit for the detection of .alpha. platelet derived growth factor receptor

US5863739A · kind A · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• William LaRochelle · Madison, US
• Jacalyn H. Pierce · Bethesda, US
• Roy A. Jensen · Westminster, US
• Stuart A. Aaronson · Great Falls, US

Key dates

• Filing date: Jun 2, 1995
• Grant date: Jan 26, 1999
• Expiry date: Jun 2, 2015

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/71
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Potent neutralizing monoclonal antibodies to the human .alpha. PDGF receptor (.alpha. PDGFR) and fragments thereof are described. These monoclonal antibodies specifically bind to an epitope on .alpha. PDGFR, inhibits PDGF binding with PDGF, antagonizes PDGF, and does not bind .beta. PDGFR receptor. A hybridoma cell line producing such a monoclonal antibody, methods of in vivo imaging of a pathological conditions and methods of inhibiting the growth of a neoplasia expressing .alpha. PDGFR, which use these monoclonal antibodies are also described. In vitro assays for detecting the presence of .alpha. PDGFR and for evaluating the binding affinity of a test compound are also described.