Method for detecting a target compound in a substance using a nucleic acid ligand
US5874218A · kind A · utility
99Cited by
5References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 5, 1996 |
| Grant date | Feb 23, 1999 |
| Priority date | — |
| Expiry date | Apr 5, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2458/10
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention discloses novel detection methods for determining the presence of a target compound in a substance using nucleic acid ligands as detection molecules. Specifically, the substance is bound to a solid support matrix, such as those used in blot procedures, and detection of the target molecule is accomplished using the affinity and specificity of nucleic acid ligands to the target molecule. The method utilized herein for identifying and preparing said nucleic acid ligands is called SELEX. The method of the present invention is additionally useful to isolate the target compounds from the various substances.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.