Method for purification of a mixture of hydroxamate derivatized protein and native protein
US5912329A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 25, 1996 |
| Grant date | Jun 15, 1999 |
| Priority date | — |
| Expiry date | Apr 25, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method for purification of a mixture of hydroxamate derivatized protein/proteins and native protein which is characterized by treating the mixture with immobilized metal and thereby adsorbing the hydroxamate derivatized protein/proteins on the immobilized-metal and recovering the native protein. The protein could be IGF-L. It also relates to a process for the production of a native protein which is characterized by expression of the protein as a fusion protein, cleavage of the fusion protein by hydroxylanine, separation of native protein from hydroxamate derivatized protein by adsorbing the hydroxamate derivatized protein on immobilized metal and directly recovering the native protein. The use of immobilized metal affinity chromatography for separation of native protein from hydroxamate derivatized protein is also claimed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.