Methods for recording the reaction history of a solid support

US5968736A · kind A · utility

Assignees

• Cold Spring Harbor Laboratory · US
• The Trustees of Columbia University in the City of New York · US

Inventors

• W. Clark Still · New York, US
• Michael Wigler · Lloyd Harbor, US
• Michael H. J. Ohlmeyer · Plainsboro, US
• Lawrence W. Dillard · Hopewell, US
• John Charles Reader · Princeton, US

Key dates

• Filing date: Jun 7, 1995
• Grant date: Oct 19, 1999
• Expiry date: Jun 7, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B70/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Encoded combinatorial chemistry is provided, where sequential synthetic schemes are recorded using organic molecules, which define choice of reactant, and stage, as the same or different bit of information. Various products can be produced in the multi-stage synthesis, such as oligomers and synthetic non-repetitive organic molecules. Conveniently, nested families of compounds can be employed as identifiers, where number and/or position of a substituent define the choice. Alternatively, detectable functionalities may be employed, such as radioisotopes, fluorescers, halogens, and the like, where presence and ratios of two different groups can be used to define stage or choice. Particularly, pluralities of identifiers may be used to provide a binary or higher code, so as to define a plurality of choices with only a few detachable tags. The particles may be screened for a characteristic of interest, particularly binding affinity, where the products may be detached from the particle or retained on the particle. The reaction history of the particles which are positive for the characteristic can be determined by the release of the tags and analysis to define the reaction history of the pa…