Lipid-nucleic acid particles prepared via a hydrophobic lipid-nucleic acid complex intermediate and use for gene transfer
US5976567A · kind A · utility
342Cited by
15References
29Claims
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Key dates
| Filing date | Jun 6, 1996 |
| Grant date | Nov 2, 1999 |
| Priority date | — |
| Expiry date | Jun 6, 2016 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K47/6911
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Novel lipid-nucleic acid particulate complexes which are useful for in vitro or in vivo gene transfer are described. The particles can be formed using either detergent dialysis methods or methods which utilize organic solvents. Upon removal of a solubilizing component (i.e., detergent or an organic solvent) the lipid-nucleic acid complexes form particles wherein the nucleic acid is serum-stable and is protected from degradation. The particles thus formed have access to extravascular sites and target cell populations and are suitable for the therapeutic delivery of nucleic acids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.