Catalytic antibody/substrate intermediates

US5985626A · kind A · utility

Assignee

• THE SCRIPPS RESEARCH INSTITUTE · US

Inventors

• Carlos F. Barbas, III · Solana Beach, US
• Richard A. Lerner · Village of La Jolla, US
• Juergen Wagner · Nittendorf, DE

Key dates

• Filing date: Mar 31, 1998
• Grant date: Nov 16, 1999
• Expiry date: Mar 31, 2018

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/901
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Antibodies that catalyze the aldol reaction are generated by immunization with a reactive compound that covalently traps a Lysine (Lys) residue in the binding pocket of the antibody by formation of a stable vinylogous amide, i.e., a covalent antibody/hapten complex. The resultant catalytic antibodies employ a catalytic mechanism which mimics the catalytic mechanism employed by natural class I aldolase enzymes.