Patent · US Expired

Homogeneous methods for nucleic acid amplification and detection

US5994056A · kind A · utility

597Cited by
7References
21Claims
0Family size

Assignee

Inventor

Key dates

Filing dateMay 2, 1991
Grant dateNov 30, 1999
Priority date—
Expiry dateMay 2, 2011

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/143333
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

This invention relates to improved methods for nucleic acid detection using methods such as the polymerase chain reaction (PCR). More specifically, the invention provides methods for simultaneous amplification and detection to enhance the speed and accuracy of prior methods. The methods involve the introduction of detectable DNA binding agents into the amplification reaction, which agents produce a detectable signal that is enhanced upon binding double-stranded DNA. In a preferred embodiment, the binding agent is a fluorescent dye. The methods also provide means for monitoring the increase in product DNA during an amplification reaction.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.