Inhibition of protein kinase activity by aptameric action of oligonucleotides
US5998596A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 4, 1995 |
| Grant date | Dec 7, 1999 |
| Priority date | — |
| Expiry date | Apr 4, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/321
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention are oligonucleotides that specifically bind to and directly inhibit the biological function of target molecules such as proteins, peptides or derivatives. The direct or aptameric interaction of oligonucleotides of the present invention with proteins, peptides and derivatives represents a non-antisense mediated effect. The oligonucleotides have been shown to bind to isolated target molecules and to inhibit biological function of the target molecule within cells. In particular, the oligonucleotides have been shown to directly inhibit the kinase activity of protein-tyrosine kinase. The oligonucleotides of the present invention have significant beneficial effects against a chronic myelogenous leukemia derived cell line as demonstrated using cellular phosphotyrosine content as well as cellular growth in soft agar.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.