Cytoplasmic expression of antibodies, antibody fragments and antibody fragment fusion proteins in E. coli

US6008023A · kind A · utility

Assignee

• Hoechst Marion Roussel Deutschland GmbH · DE

Inventors

• Martin Opper · Marburg, DE
• Klaus Bosslet · Marburg, DE
• Joerg Czech · Marburg, DE

Key dates

• Filing date: Apr 10, 1996
• Grant date: Dec 28, 1999
• Expiry date: Apr 10, 2016

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/849
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention relates to the cytoplasmic expression of antibodies, antibody fragments and antibody fragment fusion molecules in E. coli. In particular, antibody fragment fusion molecules having an antibody moiety which is directed against tumors and an enzyme moiety which cleaves a nontoxic prodrug to give the toxic drug can be advantageously prepared in this way while retaining their respective functional properties.