Crossless retroviral vectors
US6013517A · kind A · utility
28Cited by
9References
38Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 5, 1997 |
| Grant date | Jan 11, 2000 |
| Priority date | — |
| Expiry date | May 5, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/13052
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Retroviral vector constructs are described which have a 5' LTR, a tRNA binding site, a packaging signal, one or more heterologous sequences, an origin of second strand synthesis and a 3' LTR, wherein the vector construct lacks retroviral gag/pol or env coding sequences. In addition, gag/pol, and env expression-cassettes are described wherein the expression cassettes lack a consecutive sequence of more than 8 nucleotides in common. The above-described retroviral vector constructs, gag/pol and env expression cassettes may be utilized to construct producer cell lines which preclude the formation of replication competent virus.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.