Laminated membrane structure for polarographic measurement and methods of making said structures
US6020052A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jan 28, 1998 |
| Grant date | Feb 1, 2000 |
| Priority date | — |
| Expiry date | Jan 28, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T428/249987
- WIPO fieldSurface technology, coating
- WIPO sectorChemistry
Abstract
An improved laminated enzyme containing membrane for polarographic sensors and methods of making these membranes are disclosed. The laminated membrane comprises an outer, support layer having pores therein of greater than about 300 angstrom units in diameter and this outer membrane has a pore density of less than 6.times.10.sup.8 pores/cm.sup.2. The porosity of the outer membrane may be on the order of 0.001-0.2%. An enzyme layer is interposed between the support layer and an inner, barrier layer; the latter positioned near the working electrode of the polarographic cell. The enzyme layer comprises a buffer solution dispersed therein wherein the molarity of the buffer solution is between about 1.times.10.sup.-6 to 0.1 M. The enzyme layer is highly dense as measured by a molar response ratio of ferrocyanide:H.sub.2 O.sub.2 of less than about 0.05.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.