Interactions between RaF proto-oncogenes and CDC25 phosphatases, and uses related thereto
US6037136A · kind A · utility
29Cited by
3References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 24, 1994 |
| Grant date | Mar 14, 2000 |
| Priority date | — |
| Expiry date | Oct 24, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/42
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention derives from the discovery that CDC25 phosphatases and Raf proteins are able to physically interact to form protein-protein complexes, with the Raf protein mediating the activation of CDC25 phosphatases. The present invention provides both cell-free and cellular assays for detecting agents which modulate the ras-dependent activation of CDC25, as for example, by affecting the binding of a CDC25 protein with Raf, or Raf-associated complexes. Also disclosed is a method for transforming/immortalizing cells, particularly primary cell cultures.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.