Patent · US Expired

In vitro diagnostic assay employing HIV-2 antigens for the detection of HIV-2 specific antibodies

US6048685A · kind A · utility

16Cited by
6References
3Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJun 6, 1995
Grant dateApr 11, 2000
Priority date
Expiry dateJun 6, 2015

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S435/974
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

The molecular cloning and characterization of a novel human immunodeficiency virus type 2 (HIV-2), designated HIV-2.sub.ROD, is described. A recombinant .lambda. phage genomic library was screened with an HIV-2-specific probe to identify overlapping subgenomic clones. Fragments of these .lambda. phage clones were subcloned into a suitable vector to reconstitute the complete HIV-2.sub.ROD genome. The complete nucleotide sequence of this proviral clone was ascertained and the following genes and gene products identified: gag (including p16, p26, and p12), pol, vif, vpr, vpx, env, tat, rev, and nef. These gene products will be useful, inter alia, in in vitro diagnostic methods and kits for the detection of HIV-2-specific antisera.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.