Process for aligning macromolecules on a surface by passage through a meniscus
US6054327A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Mar 23, 1998 |
| Grant date | Apr 25, 2000 |
| Priority date | — |
| Expiry date | Mar 23, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/145555
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Macromolecules such as nucleic acids, proteins, lipids or polysaccharides are aligned on a support surface by passing the macromolecules through a meniscus of a solvent containing the macromolecules. The meniscus may be that of a solvent between two surfaces at an interface of the solvent with air. One end of a macromolecule is attached to one surface which may be a glass surface and another end is free. The meniscus is moved relative to the surface to which the end is attached such as by evaporating the solvent or by moving the surface. As the macromolecule passes through the meniscus, the macromolecule aligns on the surface perpendicular to the meniscus. This method may be used in assaying, measuring intramolecular distance and/or separating of macromolecules.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.