Systematic evolution of ligands exponential enrichment: blended selex
US6083696A · kind A · utility
106Cited by
1References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 23, 1997 |
| Grant date | Jul 4, 2000 |
| Priority date | — |
| Expiry date | Oct 23, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2541/101
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is described for generating blended nucleic acid ligands containing non-nucleic acid functional units. Specifically, a SELEX identified RNA ligand to the integrin gpIIbIIIa is conjugated to the peptide Gly-Arg-Gly-Asp-Thr-Pro (SEQ ID NO:1). This blended RNA ligand inhibits the biological activity of gpIIbIIIa with high specificity. Also described is a single-stranded DNA ligand to elastase coupled to N-methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl (SEQ ID NO:3) ketone. This elastase blended nucleic acid ligand inhibits the biological activity of elastase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.