Preparation of lipid-nucleic acid particles using a solvent extraction and direct hydration method
US6110745A · kind A · utility
28Cited by
2References
52Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 23, 1998 |
| Grant date | Aug 29, 2000 |
| Priority date | — |
| Expiry date | Jul 23, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2320/32
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a novel Solvent Extraction and Direct Hydration (SEDH) method for preparing lipid-nucleic acid particles which are useful for the introduction of nucleic acids (e.g., plasmid DNA, antisense molecules, ribozymes, etc.) into cells. The lipid-nucleic acid particles prepared using the methods of the present invention have enhanced circulation characteristics and serum stability and, thus, they are extremely effective as nucleic acid delivery vehicles.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.