High level expression of proteins

US6114148A · kind A · utility

Assignee

• The General Hospital Corporation · US

Inventors

• Brian Seed · Boston, US
• Jurgen Haas · Schriesheim, DE

Key dates

• Filing date: Sep 20, 1996
• Grant date: Sep 5, 2000
• Expiry date: Sep 20, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2740/16122
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention features a synthetic gene encoding a protein normally expressed in a mammalian cell wherein at least one non-preferred or less preferred codon in the natural gene encoding the protein has been replaced by a preferred codon encoding the same amino acid.