Method for screening for inhibitors and activators of type III secretion machinery in gram-negative bacteria
US6136542A · kind A · utility
7Cited by
0References
16Claims
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Key dates
| Filing date | May 7, 1999 |
| Grant date | Oct 24, 2000 |
| Priority date | — |
| Expiry date | May 7, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/01
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to mutant strains of gram-negative bacteria that constitutively secrete proteins via the type III secretion machinery. It also relates to methods of identifying molecules that are able to activate or inhibit secretion in wild-type strains of gram-negative bacteria by exposing gram-negative bacterial cells to a sample molecule, wherein said bacterial cells contain a reporter gene transcriptionally fused to a promoter of a gene activated or regulated by the type III secretion machinery, and detecting the presence or activity of the product of the reporter gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.