Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
US6177555A · kind A · utility
42Cited by
5References
4Claims
0Family size
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Key dates
| Filing date | Nov 23, 1999 |
| Grant date | Jan 23, 2001 |
| Priority date | — |
| Expiry date | Nov 23, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/682
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The presence of a target in a test mixture or the concentration of such target can be determined by a method relying on fluorescence emission measurement. Such method utilizes a nucleic acid ligand to the target and a ligand beacon comprising: i) a nucleic acid sequence complimentary to at least a portion of said nucleic acid ligand, ii) a fluorescent group, and iii) a fluorescence-modifying group. The emission profile of the ligand beacon is altered based on the presence and/or concentration change of the target in the test mixture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.