Polymerase enhancing factor (PEF) extracts PEF protein complexes isolated PEF proteins and methods for purifying and identifying same
US6183997A · kind A · utility
15Cited by
4References
41Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Mar 21, 1997 |
| Grant date | Feb 6, 2001 |
| Priority date | — |
| Expiry date | Mar 21, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Novel extracts, proteins, and complexes are identified, purified, and analyzed, which improve the polymerization activity of nucleic acid polymerases. Included within the aspects of the invention are methods for identifying compositions with a polymerase enhancing activity, methods for purifying and using these compositions, and specific extracts, proteins, and complexes that function to enhance polymerase activity. Implications for numerous assays and techniques are described. For example, the invention can be used to enhance polymerase activity in a PCR process and to increase the sensitivity of a PCR-based assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.