Neutralization of food allergens by thioredoxin

US6190723A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• Bob B. Buchanan · Berkeley, US
• Karoly Kobrehel · Montpellier, FR
• Boihon C. Yee · Walnut Creek, US
• Rosa Lozano · Madrid, ES
• Oscar Frick · San Francisco, US
• Richard W. Ermel · Winters, US

Key dates

• Filing date: Mar 23, 1998
• Grant date: Feb 20, 2001
• Expiry date: Mar 23, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y108/01009
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Thioredoxin, a small dithiol protein, is a specific reductant for major allergenic proteins present in widely used foods from animal and plant sources. All targeted allergenic proteins contain disulfide (S--S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active in the oxidized (S--S) state. When reduced (SH state), they lose their allergenicity. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by dithiothreitol, a chemical reductant. Skin tests and feeding experiments carried out with sensitized dogs showed that treatment of the food with reduced thioredoxin prior to ingestion eliminated or decreased the allergenicity of the food.