Stable transglutaminase preparations and processes for their production

US6204036A · kind A · utility

Assignee

• Aventis Behring GmbH · DE

Inventors

• Hubert Metzner · Marburg, DE
• Hermann E. Karges · Marburg, DE

Key dates

• Filing date: Dec 22, 1997
• Grant date: Mar 20, 2001
• Expiry date: Dec 22, 2017

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61P17/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention relates to stable preparation forms of a transglutaminase, for example factor XIII, which, after lyophilization, are readily soluble without turbidity. The preparation froms comprise, in addition to the transglutaminase additives selected from the group consisting of D- and/or L-amino acids other than glycine and arginine, their salts, derivatives and homologs, or dimers or oligomers thereof or mixtures thereof, and sugars or sugar alcohols. The formulations may also comprise surface active agents and/or reducing agents. The invention provides processes for preparing stable protein preparations and to the use of the described stable preparation forms for producing pharmaceuticals which are suitable, for example, for treating diseases which are characterized by F XIII deficiency.