Process for the production of DNA comprising the genome of the hepatitis B virus and vector including it
US6225458A · kind A · utility
0Cited by
4References
30Claims
0Family size
Assignees
Inventors
Key dates
| Filing date | Nov 23, 1994 |
| Grant date | May 1, 2001 |
| Priority date | — |
| Expiry date | Nov 23, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2730/10122
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention relates to a process for producing DNA corresponding to that of the DNA of the virus of B hepatitis. It comprises cloning in bacteria the latter DNA, previously repaired by means of the corresponding precursor nucleotides in the presence of a polymerase. The invention also relates to vectors containing said cloned DNA in their genomes. The cloned DNA is useful as a probe for detecting the presence of the virus of B hepatitis in biological samples, particularly blood or plasma. Its expression in bacteria provides a hybrid protein containing a protein fragment having vaccinating properties against hepatitis B.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.