Methods for structure analysis of oligosaccharides

US6228654A · kind A · utility

Assignees

• THE SCRIPPS RESEARCH INSTITUTE · US
• The Rockefeller University · US

Inventors

• Brian T. Chait · New York, US
• Ying Zhao · New York, US
• Stephen B. H. Kent · Melbourne, AU

Key dates

• Filing date: May 9, 1996
• Grant date: May 8, 2001
• Expiry date: May 9, 2016

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/25125
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method of increasing the sensitivity and efficiency of MALDI-MS analysis of an oligosaccharide which comprises derivatization, prior to analysis by MALDI-MS, of said oligosaccharide by efficient ligation to a basic aminooxyacetylpeptide by oxime formation reaction to result in the formation of a glycoconjugate.