cDNA and genomic clones encoding human .mu. opiate receptor and the purified gene product
US6258556A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 28, 1994 |
| Grant date | Jul 10, 2001 |
| Priority date | — |
| Expiry date | Jan 28, 2014 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A human .mu. opiate receptor cDNA has been identified from a cerebral cortical CDNA library using sequences from the rat .mu. opiate receptor CDNA. The human .mu. opiate receptor (h.mu.OR1) shares 95% amino acid identity with the rat sequence. The expressed .mu.OR1 recognizes tested opiate drugs and opioid peptides in a sodium- and GTP-sensitive fashion with affinities virtually identical to those displayed by the rat .mu. opiate receptor. Effects on cyclic AMP are similar to those noted for the rat .mu. opiate receptor. Overlapping genomic clones spanning 50 kilobasepairs and hybridizing with the h.mu.OR1 cDNA contains exon sequences encoding the entire open reading frame of the human A opiate receptor are described. Analysis of hybridization to DNA prepared from human rodent hybrid cell lines and chromosomal in situ hybridization studies indicate localization to 6q24-25. An MspI polymorphism, producing a 3.7 kb band, is being used to assess this gene's involvement in neuropsychiatric disorders involving opiatergic systems.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.