Template-dependent ligation with PNA-DNA chimeric probes
US6297016A · kind A · utility
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Key dates
| Filing date | Oct 8, 1999 |
| Grant date | Oct 2, 2001 |
| Priority date | — |
| Expiry date | Oct 8, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6862
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods, kits, and compositions for ligation of PNA-DNA chimeric probes and oligonucleotides when they are hybridized adjacently to template nucleic acids using ligases and ligation reagents. Structural requirements of the chimeras for ligation include 5 to 15 contiguous PNA monomer units, 2 or more contiguous nucleotides, and a 3' hydroxyl or 5' hydroxyl terminus. The chimera and/or oligonucleotide may be labelled with fluorescent dyes or other labels. The methods include, for example, oligonucleotide-ligation assays (OLA) and single nucleotide polymorphism detection.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.