General chemical ligation

US6307018A · kind A · utility

Assignee

• THE SCRIPPS RESEARCH INSTITUTE · US

Inventors

• Stephen B. H. Kent · Melbourne, AU
• Tom W. Muir · New York, US
• Philip E. Dawson · Solana Beach, US
• Steven Bark · San Diego, US
• Lynne Canne Bannen · Pacifica, US

Key dates

• Filing date: Jun 22, 1999
• Grant date: Oct 23, 2001
• Expiry date: Jun 22, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K1/04
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein is a process whereby oligopeptides are ligated to form ligation peptide products. In the first step, two starting oligopeptides are ligated to form an intermediate having an amino-thioester linkage. In the second step, the aminothioester linkage undergoes a rearrangement to form a peptide having an N-substituted amide linkage. In an optional third step, the N-substitution of the amide linkage is chemically removed to form a native peptide linkage.