Transformation of guar
US6307127A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 4, 1997 |
| Grant date | Oct 23, 2001 |
| Priority date | — |
| Expiry date | Apr 4, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8205
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method for the transformation of legumes of the genus Cyamopsis, in particular Agrobacterium-mediated transformation of guar (Cyamopsis tetragonoloba), by introducing a recombinant DNA sequence into at least one cell or protoplast and generating genetically modified explants using at least one selection or shoot growth medium comprising at least one compound selected from an auxin inhibitor, e.g. 2-(p-chlorophenoxy)-2-methylpropionic acid (PCIB), a .beta.-lactamase inhibitor, e.g. sulbactam, and an ethylene inhibitor, e.g. silver thiosulfate, so as to obtain genetically modified plant or part thereof containing in its genome at least one recombinant DNA sequence; to genetically modified plants produced by the method; and to the use of substances such as the .beta.-lactamase inhibitor sulbactam to facilitate transformation of guar and other plants.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.