Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same

US6309823A · kind A · utility

Assignee

• AFFYMETRIX, INC. · US

Inventors

• Maureen T. Cronin · Los Altos, US
• Charles Miyada · Sunnyvale, US
• Earl Hubbell · Mountain View, US
• Mark S. Chee · Encinitas, US
• Stephen P. A. Fodor · Palo Alto, US
• Xiaohua Huang · Qingdao, CN
• Robert J. Lipshutz · Palo Alto, US
• Peter E. Lobban · Mountain View, US
• MacDonald Morris · Felton, US
• Edward L. Sheldon · Oakland, US

Key dates

• Filing date: Jan 3, 1997
• Grant date: Oct 30, 2001
• Expiry date: Jan 3, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B60/14
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides arrays of immobilized probes, and methods employing the arrays, for detecting mutations in the biotransformation genes, such as cytochromes P450. For example, one such array comprises four probe sets. A first probe set comprises a plurality of probes, each probe comprising a segment of at least three nucleotides exactly complementary to a subsequence of a reference sequence from a biotransformation gene, the segment including at least one interrogation position complementary to a corresponding nucleotide in the reference sequence. Second, third and fourth probe sets each comprise a corresponding probe for each probe in the first probe set. The probes in the second, third and fourth probe sets are identical to a sequence comprising the corresponding probe from the first probe set or a subsequece of at least three nucleotides thereof that includes the at least one interrogation position, except that the at least one interrogation position is occupied by a different nucleotide in each of the four corresponding probes from the four probe sets.