Polymerase extension at 3' terminus of PNA-DNA chimera
US6316230A · kind A · utility
75Cited by
2References
43Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 13, 1999 |
| Grant date | Nov 13, 2001 |
| Priority date | — |
| Expiry date | Aug 13, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods and a kit for primer extension of PNA-DNA chimera from template nucleic acids using polymerases, nucleotide 5'-triphosphates, and primer extension reagents. Structural requirements of the chimera for primer extension include 5 to 15 contiguous PNA monomer units, 3 or more contiguous nucleotides, and a 3' hydroxyl terminus. The chimera and/or a nucleotide is labelled with fluorescent dyes or other labels. The methods include DNA sequencing, DNA fragment analysis, reverse transcription, mini-sequencing, chromosome labelling, amplification, and single nucleotide polymorphism (SNP) detection.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.