"Modified rapid expansion methods (""modified-REM"") for in vitro propagation of T lymphocytes"
US6316257A · kind A · utility
23Cited by
7References
26Claims
0Family size
Assignee
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Key dates
| Filing date | Mar 3, 1997 |
| Grant date | Nov 13, 2001 |
| Priority date | — |
| Expiry date | Mar 3, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2502/11
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides a modified rapid expansion method (termed "low-PBMC-REM" or "modified-REM"), for quickly generating large numbers of T lymphocytes, including cytolytic and helper T lymphocytes, without using the large excesses of peripheral blood mononuclear cells (PBMC) or EBV-transformed lymphoblastoid cells (LCL) characteristic of high-PBMC-REM. Clonal expansions of greater than 500-fold can be achieved within a single stimulation cycle of about 8-14 days.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.