Pigment epithelium-derived factor: characterization, genomic organization and sequence of PEDF gene

US6319687A · kind A · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Gerald Chader · Bethesda, US
• S. Patricia Becerra · Bethesda, US
• Joyce Tombran-Tink · Parkville, US
• Lincoln V. Johnson · Lompoc, US
• Fintan R. Steele · Washington, US
• Ignacio R. Rodriguez · Rockville, US

Key dates

• Filing date: Dec 30, 1994
• Grant date: Nov 20, 2001
• Expiry date: Dec 30, 2014

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Nucleic acids encoding the neurotrophic protein known as pigment epithelium-derived factor (PEDF), a truncated version of PEDF referred to as rPEDF, and equivalent proteins, vectors comprising such nucleic acids, host cells into which such vectors have been introduced, recombinant methods for producing PEDF, rPEDF, and equivalent proteins, the rPEDF protein and equivalent proteins of rPEDF and PEDF-BP, -BX and BA, and the PEDF protein produced by recombinant methods. Effects and uses of these variants on 1) neuronal differentiation (neurotrophic effect) 2) neuron survival (neuronotrophic effect) and 3) glial inhibition (gliastatic effect) are described.