Production of fucosylated carbohydrates by enzymatic fucosylation synthesis of sugar nucleotides; and in situ regeneration of GDP-fucose
US6319695A · kind A · utility
36Cited by
15References
20Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 14, 1992 |
| Grant date | Nov 20, 2001 |
| Priority date | — |
| Expiry date | Oct 14, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/26
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
This invention contemplates improved methods of enzymatic production of carbohydrates especially fucosylated carbohydrates. Improved syntheses of glycosyl 1- or 2-phosphates using both chemical and enzymatic means are also contemplated. The phosphorylated glycosides are then used to produce sugar nucleotides that are in turn used as donor sugars for glycosylation of acceptor carbohydrates. Especially preferred herein is the use of a disclosed method for fucosylation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.