Method of solubilizing, purifying, and refolding protein
US6319896A · kind A · utility
6Cited by
3References
24Claims
0Family size
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Key dates
| Filing date | Nov 18, 1999 |
| Grant date | Nov 20, 2001 |
| Priority date | — |
| Expiry date | Nov 18, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/8114
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of modifying protein solubility employs polyionic polymers. These facilitate the solubilization, formulation, purification and refolding of proteins especially incorrectly folded proteins and aggregated proteins. Compositions are described that are suitable for formulating TFPI. The compositions allow preparation of pharmaceutically acceptable compositions of TFPI at concentrations above 0.2 mg/mL and above 10 mg/mL.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.