Method of solubilizing, purifying, and refolding protein
US6323326A · kind A · utility
13Cited by
5References
69Claims
0Family size
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Key dates
| Filing date | Jun 11, 1999 |
| Grant date | Nov 27, 2001 |
| Priority date | — |
| Expiry date | Jun 11, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/8114
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of modifying protein solubility employs polyionic polymers. These facilitate the solubilization, formulation, purification and refolding of proteins especially incorrectly folded proteins and aggregated proteins. Compositions are described that are suitable for formulating TFPI. The compositions allow preparation of pharmaceutically acceptable compositions of TFPI at concentrations above 0.2 mg/mL and above 10 mg/mL.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.