Compositions and methods for packaging of alphavirus vectors

US6329201A · kind A · utility

Assignee

• Chiron Corporation · US

Inventors

• John Polo · Hayward, US
• Thomas W. Dubensky, Jr. · Piedmont, US
• Stephen F. Hardy · San Francisco, US
• Silvia Perri · Castro Valley, US

Key dates

• Filing date: Jun 30, 2000
• Grant date: Dec 11, 2001
• Expiry date: Jun 30, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/60
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Nucleic acid molecule are provided comprising a nucleic acid sequence which encodes, in order, an alphavirus capsid, a signal peptide, and an alphavirus E1 or E2 glycoprotein. Also provided are vectors encoding such nucleic acid molecules, and use of such vectors or expression cassettes to generate recombinant alphavirus particles and alphavirus packaging cell lines. In addition, modified alphavirus vector constructs are provided that permit reduced transgene expression during vector packaging, as well as methods of using such vector constructs for the production of alphavirus vector particles.