DNA construct comprising a vector for expression of human cytoplasmic Cu/Zn superoxide dismutase in E. coli and method of use of same
US6331421A · kind A · utility
2Cited by
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8Claims
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Key dates
| Filing date | Jan 19, 1995 |
| Grant date | Dec 18, 2001 |
| Priority date | — |
| Expiry date | Jan 19, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions are provided for the production of human superoxide dismutase and a novel protocol for enhancing efficiency of expression. The gene encoding for human superoxide dismutase is isolated and inserted into a vector in conjunction with a synthetic linker which provides for enhanced efficiency in translation. E. coli strain D1210 (pSODX8) was deposited at the A.T.C.C. on Sep. 27, 1983 and given Accession No. 39453. Yeast strain 2150-2-3 (pC1/1GAPSOD) and E. coli strains D1210 (pSOD11) and D1210 (pS20R) were deposited at the A.T.C.C. on May 9, 1984, and given Accession Nos. 20708, 39679 and 39,680, respectively.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.