Crossless retroviral vectors

US6333195A · kind A · utility

Assignee

• Chiron Corporation · US

Inventors

• James G. Respess · San Diego, US
• Nicholas J. DePolo · Solana Beach, US
• Sunil Chada · Vista, US
• Sybille Sauter · Del Mar, US
• Mordechai Bodner · San Diego, US
• David A. Driver · San Diego, US

Key dates

• Filing date: Jan 7, 2000
• Grant date: Dec 25, 2001
• Expiry date: Jan 7, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2740/13052
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Retroviral vector constructs are described which have a 5' LTR, a tRNA binding site, a packaging signal, one or more heterologous sequences, an origin of second strand synthesis and a 3' LTR, wherein the vector construct lacks retroviral gag/pol or env coding sequences. In addition, gag/pol, and env expression cassettes are described wherein the expression cassettes lack a consecutive sequence of more than 8 nucleotides in common. The above-described retroviral vector constructs, gag/pol and env expression cassettes may be utilized to construct producer cell lines which preclude the formation of replication competent virus.