Patent · US Expired

Multiplexing methods for identifying nucleic acids using denaturing liquid chromatography

US6379889B1 · kind B1 · utility

5Cited by
7References
19Claims
0Family size

Assignee

Inventors

Key dates

Filing dateNov 4, 1999
Grant dateApr 30, 2002
Priority date
Expiry dateNov 4, 2019

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/143333
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A method is provided for the separation of nucleic acid samples. The method includes: providing a test mixture of a plurality of nucleic acid samples, wherein each sample is labeled with a spectrally detectable tag; applying the test mixture to a reversed phase solid support; eluting the mixture under partially denaturing conditions; and detecting spectrally resolved signals produced by the nucleic acid samples labeled with the detectable tags.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.