Synthesis and purification of hepatitis C virus-like particles

US6387662B1 · kind B1 · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• T. Jake Liang · Potomac, US
• Thomas Baumert · Glückstadt, DE

Key dates

• Filing date: Apr 21, 1999
• Grant date: May 14, 2002
• Expiry date: Apr 21, 2019

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S977/802
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Production of enveloped RNA virus-like particles intracellularly in vitro in insect cells using a recombinant baculovirus vector containing a cDNA coding for viral structural proteins is disclosed. In vitro production and purification of hepatitis C virus (HCV)-like particles containing HCV core protein, E1 protein and E2 protein is disclosed. Production of antibodies in vivo to the purified HCV-like particles is disclosed.