Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction

US6399320B1 · kind B1 · utility

Assignee

• Roche Molecular Systems, Inc. · US

Inventors

• Ursula Markau · Polling, DE
• Christine Ebenbichler · Antdorf, DE
• Gunthar Achhammer · Penzberg, DE
• Waltraud Ankenbauer · Penzberg, DE

Key dates

• Filing date: Dec 1, 1998
• Grant date: Jun 4, 2002
• Expiry date: Dec 1, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/1252
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A purified DNA polymerase exhibiting reverse transcriptase activity in the presence of magnesium ions and/or manganese ions having reduced or no 5′-3′-exonuclease activity and substantially no RNaseH activity and obtainable from Carboxydothermus hydrogenoformans.