Method for the affinity isolation of newly synthesized RNA
US6413720B1 · kind B1 · utility
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14Claims
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Key dates
| Filing date | Jan 11, 1999 |
| Grant date | Jul 2, 2002 |
| Priority date | — |
| Expiry date | Jan 11, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6809
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for the isolation of newly synthesized mRNA. Such methods involve the incorporation of biotinylated rNTP analogues into cellular mRNA, and separating biotinylated mRNA from unlabeled RNA. The methods provided herein may be used, for example, for gene discovery, drug screens and studies of the regulation of gene expression.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.