Binary probe and clamp composition and methods for a target hybridization detection
US6432642B1 · kind B1 · utility
23Cited by
10References
39Claims
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Key dates
| Filing date | Jan 15, 1999 |
| Grant date | Aug 13, 2002 |
| Priority date | — |
| Expiry date | Jan 15, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6818
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end-point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.