Methods for detecting intermolecular interactions in vivo and in vitro
US6444421B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Apr 2, 1998 |
| Grant date | Sep 3, 2002 |
| Priority date | — |
| Expiry date | Apr 2, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6897
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods for assessing intermolecular interactions in vivo and in vitro are provided. Methods are provided for detecting protein-DNA interactions in vivo, in which a cell having a chimeric guide endonuclease molecule and a target nucleic acid is provided, and cleavage of the target nucleic acid by the chimeric guide endonuclease molecule is monitored. Cleavage by the chimeric guide molecule corresponds to binding of the guide domain to the target nucleic acid, or to a protein associated with the nucleic acid. The methods of the invention are adapted to cleavage of target nucleic acids, amplification of target nucleic acids, detection of target nucleic acids, screening of genomic target nucleic acid sequences for guide binding domains, and screening for modulators of chimeric guide binding domain activity. Also provided are methods for detecting interactions between other molecules, including hormones and receptors, enzymes and substrates, and the like.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.